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krt20 cre tdtomato lines  (Shanghai Model Organisms Center)
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Shanghai Model Organisms Center krt20 cre tdtomato lines
Krt20 deficient cytokeratin network leads to larger umbrella cell size (A) SMI imaged the mesh-like 3D-structure of Krt20-dependent cytokeratin network in umbrella cell apical surface. a. zoom-in region. (B) Schematic <t>for</t> <t>generating</t> <t>Krt20-Cre-tdTomato</t> mouse line. (C) RT-qPCR analysis showed the mRNA levels of Krt20 and Tjp1 in the bladders of Krt20 mutant mice. ( n = 6 mice for each group). Data are represented as mean ± SEM. Unpaired Student’s t test, ∗∗∗∗, p < 0.0001. (D) Krt20 mutant mice lost KRT20 expression. Scale bar, 20 μm. U, Urothelium; LP, lamina propria. (E and F) Krt20-tdTomato in situ tracing shown that KRT20 protein restricted in urinary tract (E) and enriched in umbrella cell (F). Scale bar in (E), 2 mm. Scale bar in (F), 20 μm. BL, bladder; Ure, ureter; Kd, kidney, U, urothelium; LP, lamina propria. (G–I) Micro-dissected urothelium image (G) shown larger umbrella cell size (H and I) in Krt20 mutant mice. ( n = 6 mice for each group). Scale bars, 200 μm. For (H), each dot represents an umbrella cell. Data are represented as mean ± SEM. Unpaired Student’s t test, ∗, p < 0.05, ∗∗∗∗, p < 0.0001.
Krt20 Cre Tdtomato Lines, supplied by Shanghai Model Organisms Center, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse krt20 cre tdtomato  (Shanghai Model Organisms Center)
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Shanghai Model Organisms Center mouse krt20 cre tdtomato
Krt20 deficient cytokeratin network leads to larger umbrella cell size (A) SMI imaged the mesh-like 3D-structure of Krt20-dependent cytokeratin network in umbrella cell apical surface. a. zoom-in region. (B) Schematic <t>for</t> <t>generating</t> <t>Krt20-Cre-tdTomato</t> mouse line. (C) RT-qPCR analysis showed the mRNA levels of Krt20 and Tjp1 in the bladders of Krt20 mutant mice. ( n = 6 mice for each group). Data are represented as mean ± SEM. Unpaired Student’s t test, ∗∗∗∗, p < 0.0001. (D) Krt20 mutant mice lost KRT20 expression. Scale bar, 20 μm. U, Urothelium; LP, lamina propria. (E and F) Krt20-tdTomato in situ tracing shown that KRT20 protein restricted in urinary tract (E) and enriched in umbrella cell (F). Scale bar in (E), 2 mm. Scale bar in (F), 20 μm. BL, bladder; Ure, ureter; Kd, kidney, U, urothelium; LP, lamina propria. (G–I) Micro-dissected urothelium image (G) shown larger umbrella cell size (H and I) in Krt20 mutant mice. ( n = 6 mice for each group). Scale bars, 200 μm. For (H), each dot represents an umbrella cell. Data are represented as mean ± SEM. Unpaired Student’s t test, ∗, p < 0.05, ∗∗∗∗, p < 0.0001.
Mouse Krt20 Cre Tdtomato, supplied by Shanghai Model Organisms Center, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rosa lsl tdtomato mice  (Shanghai Model Organisms Center)
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Shanghai Model Organisms Center rosa lsl tdtomato mice
Krt20 deficient cytokeratin network leads to larger umbrella cell size (A) SMI imaged the mesh-like 3D-structure of Krt20-dependent cytokeratin network in umbrella cell apical surface. a. zoom-in region. (B) Schematic <t>for</t> <t>generating</t> <t>Krt20-Cre-tdTomato</t> mouse line. (C) RT-qPCR analysis showed the mRNA levels of Krt20 and Tjp1 in the bladders of Krt20 mutant mice. ( n = 6 mice for each group). Data are represented as mean ± SEM. Unpaired Student’s t test, ∗∗∗∗, p < 0.0001. (D) Krt20 mutant mice lost KRT20 expression. Scale bar, 20 μm. U, Urothelium; LP, lamina propria. (E and F) Krt20-tdTomato in situ tracing shown that KRT20 protein restricted in urinary tract (E) and enriched in umbrella cell (F). Scale bar in (E), 2 mm. Scale bar in (F), 20 μm. BL, bladder; Ure, ureter; Kd, kidney, U, urothelium; LP, lamina propria. (G–I) Micro-dissected urothelium image (G) shown larger umbrella cell size (H and I) in Krt20 mutant mice. ( n = 6 mice for each group). Scale bars, 200 μm. For (H), each dot represents an umbrella cell. Data are represented as mean ± SEM. Unpaired Student’s t test, ∗, p < 0.05, ∗∗∗∗, p < 0.0001.
Rosa Lsl Tdtomato Mice, supplied by Shanghai Model Organisms Center, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rosa26 tdtomato mice  (Jackson Laboratory)
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Jackson Laboratory rosa26 tdtomato mice
Krt20 deficient cytokeratin network leads to larger umbrella cell size (A) SMI imaged the mesh-like 3D-structure of Krt20-dependent cytokeratin network in umbrella cell apical surface. a. zoom-in region. (B) Schematic <t>for</t> <t>generating</t> <t>Krt20-Cre-tdTomato</t> mouse line. (C) RT-qPCR analysis showed the mRNA levels of Krt20 and Tjp1 in the bladders of Krt20 mutant mice. ( n = 6 mice for each group). Data are represented as mean ± SEM. Unpaired Student’s t test, ∗∗∗∗, p < 0.0001. (D) Krt20 mutant mice lost KRT20 expression. Scale bar, 20 μm. U, Urothelium; LP, lamina propria. (E and F) Krt20-tdTomato in situ tracing shown that KRT20 protein restricted in urinary tract (E) and enriched in umbrella cell (F). Scale bar in (E), 2 mm. Scale bar in (F), 20 μm. BL, bladder; Ure, ureter; Kd, kidney, U, urothelium; LP, lamina propria. (G–I) Micro-dissected urothelium image (G) shown larger umbrella cell size (H and I) in Krt20 mutant mice. ( n = 6 mice for each group). Scale bars, 200 μm. For (H), each dot represents an umbrella cell. Data are represented as mean ± SEM. Unpaired Student’s t test, ∗, p < 0.05, ∗∗∗∗, p < 0.0001.
Rosa26 Tdtomato Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transgenic nestin creer t2 tdtomato reporter mice  (Jackson Laboratory)
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Jackson Laboratory transgenic nestin creer t2 tdtomato reporter mice
Krt20 deficient cytokeratin network leads to larger umbrella cell size (A) SMI imaged the mesh-like 3D-structure of Krt20-dependent cytokeratin network in umbrella cell apical surface. a. zoom-in region. (B) Schematic <t>for</t> <t>generating</t> <t>Krt20-Cre-tdTomato</t> mouse line. (C) RT-qPCR analysis showed the mRNA levels of Krt20 and Tjp1 in the bladders of Krt20 mutant mice. ( n = 6 mice for each group). Data are represented as mean ± SEM. Unpaired Student’s t test, ∗∗∗∗, p < 0.0001. (D) Krt20 mutant mice lost KRT20 expression. Scale bar, 20 μm. U, Urothelium; LP, lamina propria. (E and F) Krt20-tdTomato in situ tracing shown that KRT20 protein restricted in urinary tract (E) and enriched in umbrella cell (F). Scale bar in (E), 2 mm. Scale bar in (F), 20 μm. BL, bladder; Ure, ureter; Kd, kidney, U, urothelium; LP, lamina propria. (G–I) Micro-dissected urothelium image (G) shown larger umbrella cell size (H and I) in Krt20 mutant mice. ( n = 6 mice for each group). Scale bars, 200 μm. For (H), each dot represents an umbrella cell. Data are represented as mean ± SEM. Unpaired Student’s t test, ∗, p < 0.05, ∗∗∗∗, p < 0.0001.
Transgenic Nestin Creer T2 Tdtomato Reporter Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tdtomato reporter mice  (Jackson Laboratory)
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Jackson Laboratory tdtomato reporter mice
Krt20 deficient cytokeratin network leads to larger umbrella cell size (A) SMI imaged the mesh-like 3D-structure of Krt20-dependent cytokeratin network in umbrella cell apical surface. a. zoom-in region. (B) Schematic <t>for</t> <t>generating</t> <t>Krt20-Cre-tdTomato</t> mouse line. (C) RT-qPCR analysis showed the mRNA levels of Krt20 and Tjp1 in the bladders of Krt20 mutant mice. ( n = 6 mice for each group). Data are represented as mean ± SEM. Unpaired Student’s t test, ∗∗∗∗, p < 0.0001. (D) Krt20 mutant mice lost KRT20 expression. Scale bar, 20 μm. U, Urothelium; LP, lamina propria. (E and F) Krt20-tdTomato in situ tracing shown that KRT20 protein restricted in urinary tract (E) and enriched in umbrella cell (F). Scale bar in (E), 2 mm. Scale bar in (F), 20 μm. BL, bladder; Ure, ureter; Kd, kidney, U, urothelium; LP, lamina propria. (G–I) Micro-dissected urothelium image (G) shown larger umbrella cell size (H and I) in Krt20 mutant mice. ( n = 6 mice for each group). Scale bars, 200 μm. For (H), each dot represents an umbrella cell. Data are represented as mean ± SEM. Unpaired Student’s t test, ∗, p < 0.05, ∗∗∗∗, p < 0.0001.
Tdtomato Reporter Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tdtomato reporter mice - by Bioz Stars, 2026-06
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mouse anti tdtomato  (Sangon Biotech)
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Sangon Biotech mouse anti tdtomato
Krt20 deficient cytokeratin network leads to larger umbrella cell size (A) SMI imaged the mesh-like 3D-structure of Krt20-dependent cytokeratin network in umbrella cell apical surface. a. zoom-in region. (B) Schematic <t>for</t> <t>generating</t> <t>Krt20-Cre-tdTomato</t> mouse line. (C) RT-qPCR analysis showed the mRNA levels of Krt20 and Tjp1 in the bladders of Krt20 mutant mice. ( n = 6 mice for each group). Data are represented as mean ± SEM. Unpaired Student’s t test, ∗∗∗∗, p < 0.0001. (D) Krt20 mutant mice lost KRT20 expression. Scale bar, 20 μm. U, Urothelium; LP, lamina propria. (E and F) Krt20-tdTomato in situ tracing shown that KRT20 protein restricted in urinary tract (E) and enriched in umbrella cell (F). Scale bar in (E), 2 mm. Scale bar in (F), 20 μm. BL, bladder; Ure, ureter; Kd, kidney, U, urothelium; LP, lamina propria. (G–I) Micro-dissected urothelium image (G) shown larger umbrella cell size (H and I) in Krt20 mutant mice. ( n = 6 mice for each group). Scale bars, 200 μm. For (H), each dot represents an umbrella cell. Data are represented as mean ± SEM. Unpaired Student’s t test, ∗, p < 0.05, ∗∗∗∗, p < 0.0001.
Mouse Anti Tdtomato, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rosa26lsl tdtomato  (Jackson Laboratory)
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Jackson Laboratory rosa26lsl tdtomato
Krt20 deficient cytokeratin network leads to larger umbrella cell size (A) SMI imaged the mesh-like 3D-structure of Krt20-dependent cytokeratin network in umbrella cell apical surface. a. zoom-in region. (B) Schematic <t>for</t> <t>generating</t> <t>Krt20-Cre-tdTomato</t> mouse line. (C) RT-qPCR analysis showed the mRNA levels of Krt20 and Tjp1 in the bladders of Krt20 mutant mice. ( n = 6 mice for each group). Data are represented as mean ± SEM. Unpaired Student’s t test, ∗∗∗∗, p < 0.0001. (D) Krt20 mutant mice lost KRT20 expression. Scale bar, 20 μm. U, Urothelium; LP, lamina propria. (E and F) Krt20-tdTomato in situ tracing shown that KRT20 protein restricted in urinary tract (E) and enriched in umbrella cell (F). Scale bar in (E), 2 mm. Scale bar in (F), 20 μm. BL, bladder; Ure, ureter; Kd, kidney, U, urothelium; LP, lamina propria. (G–I) Micro-dissected urothelium image (G) shown larger umbrella cell size (H and I) in Krt20 mutant mice. ( n = 6 mice for each group). Scale bars, 200 μm. For (H), each dot represents an umbrella cell. Data are represented as mean ± SEM. Unpaired Student’s t test, ∗, p < 0.05, ∗∗∗∗, p < 0.0001.
Rosa26lsl Tdtomato, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tdtomato  (Arigo Biolaboratories)
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Arigo Biolaboratories tdtomato
Krt20 deficient cytokeratin network leads to larger umbrella cell size (A) SMI imaged the mesh-like 3D-structure of Krt20-dependent cytokeratin network in umbrella cell apical surface. a. zoom-in region. (B) Schematic <t>for</t> <t>generating</t> <t>Krt20-Cre-tdTomato</t> mouse line. (C) RT-qPCR analysis showed the mRNA levels of Krt20 and Tjp1 in the bladders of Krt20 mutant mice. ( n = 6 mice for each group). Data are represented as mean ± SEM. Unpaired Student’s t test, ∗∗∗∗, p < 0.0001. (D) Krt20 mutant mice lost KRT20 expression. Scale bar, 20 μm. U, Urothelium; LP, lamina propria. (E and F) Krt20-tdTomato in situ tracing shown that KRT20 protein restricted in urinary tract (E) and enriched in umbrella cell (F). Scale bar in (E), 2 mm. Scale bar in (F), 20 μm. BL, bladder; Ure, ureter; Kd, kidney, U, urothelium; LP, lamina propria. (G–I) Micro-dissected urothelium image (G) shown larger umbrella cell size (H and I) in Krt20 mutant mice. ( n = 6 mice for each group). Scale bars, 200 μm. For (H), each dot represents an umbrella cell. Data are represented as mean ± SEM. Unpaired Student’s t test, ∗, p < 0.05, ∗∗∗∗, p < 0.0001.
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rosa tdtomato reporter mice  (Jackson Laboratory)
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Jackson Laboratory rosa tdtomato reporter mice
Krt20 deficient cytokeratin network leads to larger umbrella cell size (A) SMI imaged the mesh-like 3D-structure of Krt20-dependent cytokeratin network in umbrella cell apical surface. a. zoom-in region. (B) Schematic <t>for</t> <t>generating</t> <t>Krt20-Cre-tdTomato</t> mouse line. (C) RT-qPCR analysis showed the mRNA levels of Krt20 and Tjp1 in the bladders of Krt20 mutant mice. ( n = 6 mice for each group). Data are represented as mean ± SEM. Unpaired Student’s t test, ∗∗∗∗, p < 0.0001. (D) Krt20 mutant mice lost KRT20 expression. Scale bar, 20 μm. U, Urothelium; LP, lamina propria. (E and F) Krt20-tdTomato in situ tracing shown that KRT20 protein restricted in urinary tract (E) and enriched in umbrella cell (F). Scale bar in (E), 2 mm. Scale bar in (F), 20 μm. BL, bladder; Ure, ureter; Kd, kidney, U, urothelium; LP, lamina propria. (G–I) Micro-dissected urothelium image (G) shown larger umbrella cell size (H and I) in Krt20 mutant mice. ( n = 6 mice for each group). Scale bars, 200 μm. For (H), each dot represents an umbrella cell. Data are represented as mean ± SEM. Unpaired Student’s t test, ∗, p < 0.05, ∗∗∗∗, p < 0.0001.
Rosa Tdtomato Reporter Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Krt20 deficient cytokeratin network leads to larger umbrella cell size (A) SMI imaged the mesh-like 3D-structure of Krt20-dependent cytokeratin network in umbrella cell apical surface. a. zoom-in region. (B) Schematic for generating Krt20-Cre-tdTomato mouse line. (C) RT-qPCR analysis showed the mRNA levels of Krt20 and Tjp1 in the bladders of Krt20 mutant mice. ( n = 6 mice for each group). Data are represented as mean ± SEM. Unpaired Student’s t test, ∗∗∗∗, p < 0.0001. (D) Krt20 mutant mice lost KRT20 expression. Scale bar, 20 μm. U, Urothelium; LP, lamina propria. (E and F) Krt20-tdTomato in situ tracing shown that KRT20 protein restricted in urinary tract (E) and enriched in umbrella cell (F). Scale bar in (E), 2 mm. Scale bar in (F), 20 μm. BL, bladder; Ure, ureter; Kd, kidney, U, urothelium; LP, lamina propria. (G–I) Micro-dissected urothelium image (G) shown larger umbrella cell size (H and I) in Krt20 mutant mice. ( n = 6 mice for each group). Scale bars, 200 μm. For (H), each dot represents an umbrella cell. Data are represented as mean ± SEM. Unpaired Student’s t test, ∗, p < 0.05, ∗∗∗∗, p < 0.0001.

Journal: iScience

Article Title: Keratin 20 deficiency phenocopies human UAB and drives bladder fibrosis via a mechanotransduction-TGF-β axis

doi: 10.1016/j.isci.2026.115905

Figure Lengend Snippet: Krt20 deficient cytokeratin network leads to larger umbrella cell size (A) SMI imaged the mesh-like 3D-structure of Krt20-dependent cytokeratin network in umbrella cell apical surface. a. zoom-in region. (B) Schematic for generating Krt20-Cre-tdTomato mouse line. (C) RT-qPCR analysis showed the mRNA levels of Krt20 and Tjp1 in the bladders of Krt20 mutant mice. ( n = 6 mice for each group). Data are represented as mean ± SEM. Unpaired Student’s t test, ∗∗∗∗, p < 0.0001. (D) Krt20 mutant mice lost KRT20 expression. Scale bar, 20 μm. U, Urothelium; LP, lamina propria. (E and F) Krt20-tdTomato in situ tracing shown that KRT20 protein restricted in urinary tract (E) and enriched in umbrella cell (F). Scale bar in (E), 2 mm. Scale bar in (F), 20 μm. BL, bladder; Ure, ureter; Kd, kidney, U, urothelium; LP, lamina propria. (G–I) Micro-dissected urothelium image (G) shown larger umbrella cell size (H and I) in Krt20 mutant mice. ( n = 6 mice for each group). Scale bars, 200 μm. For (H), each dot represents an umbrella cell. Data are represented as mean ± SEM. Unpaired Student’s t test, ∗, p < 0.05, ∗∗∗∗, p < 0.0001.

Article Snippet: The Krt20-Cre-tdTomato lines were generated by Shanghai Model Organisms Center.

Techniques: Quantitative RT-PCR, Mutagenesis, Expressing, In Situ

Krt20 deficient cytokeratin network leads to larger umbrella cell size (A) SMI imaged the mesh-like 3D-structure of Krt20-dependent cytokeratin network in umbrella cell apical surface. a. zoom-in region. (B) Schematic for generating Krt20-Cre-tdTomato mouse line. (C) RT-qPCR analysis showed the mRNA levels of Krt20 and Tjp1 in the bladders of Krt20 mutant mice. ( n = 6 mice for each group). Data are represented as mean ± SEM. Unpaired Student’s t test, ∗∗∗∗, p < 0.0001. (D) Krt20 mutant mice lost KRT20 expression. Scale bar, 20 μm. U, Urothelium; LP, lamina propria. (E and F) Krt20-tdTomato in situ tracing shown that KRT20 protein restricted in urinary tract (E) and enriched in umbrella cell (F). Scale bar in (E), 2 mm. Scale bar in (F), 20 μm. BL, bladder; Ure, ureter; Kd, kidney, U, urothelium; LP, lamina propria. (G–I) Micro-dissected urothelium image (G) shown larger umbrella cell size (H and I) in Krt20 mutant mice. ( n = 6 mice for each group). Scale bars, 200 μm. For (H), each dot represents an umbrella cell. Data are represented as mean ± SEM. Unpaired Student’s t test, ∗, p < 0.05, ∗∗∗∗, p < 0.0001.

Journal: iScience

Article Title: Keratin 20 deficiency phenocopies human UAB and drives bladder fibrosis via a mechanotransduction-TGF-β axis

doi: 10.1016/j.isci.2026.115905

Figure Lengend Snippet: Krt20 deficient cytokeratin network leads to larger umbrella cell size (A) SMI imaged the mesh-like 3D-structure of Krt20-dependent cytokeratin network in umbrella cell apical surface. a. zoom-in region. (B) Schematic for generating Krt20-Cre-tdTomato mouse line. (C) RT-qPCR analysis showed the mRNA levels of Krt20 and Tjp1 in the bladders of Krt20 mutant mice. ( n = 6 mice for each group). Data are represented as mean ± SEM. Unpaired Student’s t test, ∗∗∗∗, p < 0.0001. (D) Krt20 mutant mice lost KRT20 expression. Scale bar, 20 μm. U, Urothelium; LP, lamina propria. (E and F) Krt20-tdTomato in situ tracing shown that KRT20 protein restricted in urinary tract (E) and enriched in umbrella cell (F). Scale bar in (E), 2 mm. Scale bar in (F), 20 μm. BL, bladder; Ure, ureter; Kd, kidney, U, urothelium; LP, lamina propria. (G–I) Micro-dissected urothelium image (G) shown larger umbrella cell size (H and I) in Krt20 mutant mice. ( n = 6 mice for each group). Scale bars, 200 μm. For (H), each dot represents an umbrella cell. Data are represented as mean ± SEM. Unpaired Student’s t test, ∗, p < 0.05, ∗∗∗∗, p < 0.0001.

Article Snippet: Mouse: Krt20-Cre-tdTomato , This paper , Shanghai Model Organisms Center, Inc.

Techniques: Quantitative RT-PCR, Mutagenesis, Expressing, In Situ